Bench Calculators

1. 01C1V1 = C2V2 solved for whichever of the four quantities is left empty
2. 02Molar, mass per volume, fold and percent concentrations, each quantity in its own unit
3. 03The diluent volume to add and the fold dilution, beside the solved quantity
4. 04A refusal, not a negative volume, when the asked-for dilution would raise the concentration
5. 05Master mix from the vendor's own published reaction setup, scaled to any reaction volume with every final concentration kept
6. 06Water made up to volume and the template added per tube, so the mix and the tube volumes are both stated
7. 07Overage as a percent of the mix, and a custom recipe in place of the vendor setup
8. 08Reaction setups from NEB, Takara Bio and QIAGEN for every catalogued polymerase
9. 09Primers and magnesium the vendor states as a concentration turned into volumes from your stock, with a warning outside the vendor range
10. 10Serial dilutions: the transfer and diluent for every tube, and each tube concentration and cumulative dilution
11. 11Mass to moles and copies for the open molecule, weighed from its own sequence

The arithmetic is checked against the vendors' own tables: NEB's 20 µl and 50 µl Phusion setups both come out of one scaling, every column of QIAGEN's magnesium table is reproduced, and the serial dilution reproduces Carolina Biological's worked example. The panel runs on @ports/bench.

Everything runs client-side. Files are parsed in the browser and projects are saved on this machine, so an unpublished sequence is never uploaded.

Why is there no water volume to type in?

Because the vendor writes it as "to volume": water is whatever the other components leave of the reaction, so it is calculated, and the recipe always adds up to the reaction volume.

Can it convert ng to pmol?

Yes, on the Mass ↔ moles tab, for the molecule you have open: its weight is computed from its sequence. A dilution itself still needs both concentrations in the same kind of unit, because converting between them needs that weight.

Why does it ask for my primer stock?

Most vendors give the primers as a final concentration, often a range, rather than a volume. The volume depends on how concentrated your stock is, so the panel works it out from the stock and the concentration you choose.